› 6ro4

Electron Microscopy
3.5Å resolution

Structure of the core TFIIH-XPA-DNA complex

Released:
DOI: 10.2210/pdb6ro4/pdb
PDB entry 6ro4 coloured by chain, front view.
PDB entry 6ro4 coloured by chain, side view.
PDB entry 6ro4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of TFIIH activation for nucleotide excision repair.
Kokic G, Chernev A, Tegunov D, Dienemann C, Urlaub H, Cramer P
OpenAccess logo Nat Commun 10 2885 (2019)
PMID: 31253769
Related structures: EMD-4970

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-120561 (preferred)
Entry contents:
7 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (9 distinct):
General transcription and DNA repair factor IIH helicase/translocase subunit XPB Chain: A
Molecule details ›
Chain: A
Length: 782 amino acids
Theoretical weight: 89.4 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P19447 (Residues: 1-782; Coverage: 100%)
Gene names: ERCC3, XPB, XPBC
Sequence domains:
General transcription factor IIH subunit 5 Chain: F
Molecule details ›
Chain: F
Length: 71 amino acids
Theoretical weight: 8.06 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q6ZYL4 (Residues: 1-71; Coverage: 100%)
Gene names: C6orf175, GTF2H5, TTDA
Sequence domains: Transcription factor TFIIH complex subunit Tfb5
General transcription and DNA repair factor IIH helicase subunit XPD Chain: B
General transcription factor IIH subunit 3 Chain: E
General transcription factor IIH subunit 2 Chain: D
General transcription factor IIH subunit 4 Chain: C
DNA repair protein complementing XP-A cells Chain: G
DNA1 Chain: J
DNA2 Chain: K

Ligands and Environments

2 bound ligands:
Ligand SF4 1 x SF4
Ligand ZN 6 x ZN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.5Å
Relevant EMDB volumes: EMD-4970
Expression system: Trichoplusia ni

Quick links

Citations

14 review citations

Different mechanisms for translocation by monomeric and hexameric helicases.
Gao et al. (2020)
13 more

17 mentions without citation

Structure and mechanism of the RNA polymerase II transcription machinery.
Schier et al. (2020)
16 more