› 6rpr

X-ray diffraction
2.26Å resolution

LEM domain of Emerin mutant T43I in complex with BAF dimer and the Igfold of the lamin A/C

Released:
DOI: 10.2210/pdb6rpr/pdb
PDB entry 6rpr coloured by chain, front view.
PDB entry 6rpr coloured by chain, side view.
PDB entry 6rpr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An Emerin LEM-Domain Mutation Impairs Cell Response to Mechanical Stress.
Essawy N, Samson C, Petitalot A, Moog S, Bigot A, Herrada I, Marcelot A, Arteni AA, Coirault C, Zinn-Justin S
Cells 8 (2019)
PMID: 31185657

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-131038 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Lamin-A/C Chain: B
Molecule details ›
Chain: B
Length: 116 amino acids
Theoretical weight: 12.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P02545 (Residues: 430-545; Coverage: 18%)
Gene names: LMN1, LMNA
Sequence domains: Lamin Tail Domain
Barrier-to-autointegration factor Chains: D, E
Molecule details ›
Chains: D, E
Length: 87 amino acids
Theoretical weight: 9.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75531 (Residues: 3-89; Coverage: 98%)
Gene names: BAF, BANF1, BCRG1
Sequence domains: Barrier to autointegration factor
Emerin Chain: G
Molecule details ›
Chain: G
Length: 43 amino acids
Theoretical weight: 5.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P50402 (Residues: 2-44; Coverage: 17%)
Gene names: EDMD, EMD, STA
Sequence domains: LEM domain

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2
Unit cell:
a: 80.2Å b: 81.49Å c: 64.88Å
α: 90° β: 121.37° γ: 90°
R-values:
R R work R free
0.198 0.196 0.244
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Recent insight into intermediate filament structure.
Eldirany et al. (2021)
5 more