› 6rps

X-ray diffraction
2.79Å resolution

X-ray crystal structure of carbonic anhydrase XII complexed with a theranostic monoclonal antibody fragment

Released:
DOI: 10.2210/pdb6rps/pdb
Model geometry
Fit model/data
PDB entry 6rps coloured by chain, front view.
PDB entry 6rps coloured by chain, side view.
PDB entry 6rps coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Biochemical and Structural Insights into Carbonic Anhydrase XII/Fab6A10 Complex.
Alterio V, Kellner M, Esposito D, Liesche-Starnecker F, Bua S, Supuran CT, Monti SM, Zeidler R, De Simone G
J Mol Biol 431 4910-4921 (2019)
PMID: 31682835

Function and Biology Details

Reaction catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-279355 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Carbonic anhydrase 12 Chains: A, B
Molecule details ›
Chains: A, B
Length: 283 amino acids
Theoretical weight: 31.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O43570 (Residues: 31-291; Coverage: 79%)
  • Best match: O43570-2 (Residues: 31-291)
Gene name: CA12
Sequence domains: Eukaryotic-type carbonic anhydrase
Fab Light chain Chains: L, M
Molecule details ›
Chains: L, M
Length: 216 amino acids
Theoretical weight: 23.57 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Fab Heavy chain Chains: H, N

Ligands and Environments

5 bound ligands:
Ligand ZN 2 x ZN
Ligand ACT 2 x ACT
Ligand SO4 2 x SO4
Ligand CD 4 x CD
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: I222
Unit cell:
a: 77.335Å b: 222.165Å c: 266.988Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.203 0.231
Expression systems:
  • Escherichia coli BL21(DE3)
  • Cricetulus griseus

Quick links

Citations

2 review citations

Post-translational modifications in tumor-associated carbonic anhydrases.
Di Fiore et al. (2022)
1 more

1 mention without citation

Inhibitory Monoclonal Antibodies and Their Recombinant Derivatives Targeting Surface-Exposed Carbonic Anhydrase XII on Cancer Cells.
Stravinskiene et al. (2020)