› 6rt9

X-ray diffraction
1.55Å resolution

Orthorhombic lysozyme grown with 300g/L sucrose

Released:
DOI: 10.2210/pdb6rt9/pdb
PDB entry 6rt9 coloured by chain, front view.
PDB entry 6rt9 coloured by chain, side view.
PDB entry 6rt9 coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Protein crystals as a key for deciphering macromolecular crowding effects on biological reactions.
Ferreira C, Pinto MF, Macedo-Ribeiro S, Pereira PJB, Rocha FA, Martins PM
Phys Chem Chem Phys 22 16143-16149 (2020)
PMID: 32638771

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, FRU
Carbohydrate polymer
1 bound ligand:
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OXFORD DIFFRACTION GEMINI
Spacegroup: P212121
Unit cell:
a: 30.065Å b: 56.091Å c: 72.455Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.228

Quick links

Citations

3 citation in other articles

Liquid-Liquid Phase Separation in the Presence of Macromolecular Crowding and State-dependent Kinetics.
Vweza et al. (2021)
2 more