Function and Biology Details
Reaction catalysed:
Cleavage of Arg-|-Ser bond in complement component C3 alpha-chain to yield C3a and C3b, and Arg-|- bond in complement component C5 alpha-chain to yield C5a and C5b.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- Thrombospondin type-1 (TSP1) repeat
- Thrombospondin type-1 (TSP1) repeat superfamily
- Serine proteases, trypsin domain
- Peptidase S1A, chymotrypsin family
- von Willebrand factor, type A
- von Willebrand factor A-like domain superfamily
- Netrin module, non-TIMP type
- Tissue inhibitor of metalloproteinases-like, OB-fold
20 more domains
Structure analysis Details
Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-133308 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (9 distinct):
Properdin
Molecule details ›
Chains: U, X
Length: 234 amino acids
Theoretical weight: 25.49 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
Sequence domains:
Length: 234 amino acids
Theoretical weight: 25.49 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
- Canonical:
P27918 (Residues: 28-255; Coverage: 52%)
Sequence domains:
Properdin
Molecule details ›
Chains: V, Y
Length: 215 amino acids
Theoretical weight: 23.93 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
Sequence domains: Thrombospondin type 1 domain
Length: 215 amino acids
Theoretical weight: 23.93 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
- Canonical: P27918 (Residues: 256-469; Coverage: 48%)
Sequence domains: Thrombospondin type 1 domain
Complement C3 beta chain
Molecule details ›
Chains: A, G
Length: 645 amino acids
Theoretical weight: 71.39 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains:
Length: 645 amino acids
Theoretical weight: 71.39 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: P01024 (Residues: 23-667; Coverage: 39%)
Sequence domains:
Complement C3b alpha' chain
Molecule details ›
Chains: B, H
Length: 915 amino acids
Theoretical weight: 104.07 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains:
Length: 915 amino acids
Theoretical weight: 104.07 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: P01024 (Residues: 749-1663; Coverage: 56%)
Sequence domains:
Complement factor B Bb fragment
Molecule details ›
Chains: J, L
Length: 505 amino acids
Theoretical weight: 57.04 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
Sequence domains:
Length: 505 amino acids
Theoretical weight: 57.04 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
- Canonical: P00751 (Residues: 260-764; Coverage: 68%)
Sequence domains:
Staphylococcal complement inhibitor
Molecule details ›
Chains: N, Q
Length: 85 amino acids
Theoretical weight: 9.82 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
Sequence domains: Staphylococcal complement inhibitor SCIN
Length: 85 amino acids
Theoretical weight: 9.82 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
- Canonical: Q931M7 (Residues: 32-116; Coverage: 100%)
Sequence domains: Staphylococcal complement inhibitor SCIN
Ligands and Environments
Experiments and Validation Details
X-ray source:
PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup:
C2
Expression systems:
- Homo sapiens
- Escherichia coli
