6rwx

Electron Microscopy
3.55Å resolution

Periplasmic inner membrane ring of the Shigella type 3 secretion system

Released:
Source organism: Shigella flexneri
Primary publication:
Cryo-EM structure of the Shigella type III needle complex.
PLoS Pathog 16 e1008263 (2020)
PMID: 32092125
Related structures: EMD-10045

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 48-mer (preferred)
PDBe Complex ID:
PDB-CPX-141045 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein MxiG Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Length: 371 amino acids
Theoretical weight: 43.05 KDa
Source organism: Shigella flexneri
UniProt:
  • Canonical: P0A221 (Residues: 1-371; Coverage: 100%)
Gene names: CP0136, mxiG
Sequence domains: Type III secretion system protein PrgH-EprH (PrgH)
Lipoprotein MxiJ Chains: a, b, c, d, e, f, g, h, i, j, k, l, m, n, o, p, q, r, s, t, u, v, w, x
Molecule details ›
Chains: a, b, c, d, e, f, g, h, i, j, k, l, m, n, o, p, q, r, s, t, u, v, w, x
Length: 241 amino acids
Theoretical weight: 27.54 KDa
Source organism: Shigella flexneri
UniProt:
  • Canonical: Q06081 (Residues: 1-241; Coverage: 100%)
Gene names: CP0139, mxiJ
Sequence domains: Secretory protein of YscJ/FliF family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.55Å
Relevant EMDB volumes: EMD-10045