› 6ryd

X-ray diffraction
1.58Å resolution

WUS-HD bound to TGAA DNA

Released:
DOI: 10.2210/pdb6ryd/pdb
PDB entry 6ryd coloured by chain, front view.
PDB entry 6ryd coloured by chain, side view.
PDB entry 6ryd coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural basis for the complex DNA binding behavior of the plant stem cell regulator WUSCHEL.
Sloan J, Hakenjos JP, Gebert M, Ermakova O, Gumiero A, Stier G, Wild K, Sinning I, Lohmann JU
Nat Commun 11 2223 (2020)
PMID: 32376862

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120583 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Protein WUSCHEL Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 76 amino acids
Theoretical weight: 8.95 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9SB92 (Residues: 34-103; Coverage: 24%)
Gene names: At2g17950, PGA6, T27K22.18, WUS
Sequence domains: Homeodomain
DNA (5'-D(*AP*GP*TP*GP*TP*AP*TP*GP*AP*AP*TP*GP*AP*AP*CP*G)-3') Chains: C, G
Molecule details ›
Chains: C, G
Length: 16 nucleotides
Theoretical weight: 4.99 KDa
Source organism: Arabidopsis thaliana
Expression system: Not provided
DNA (5'-D(*CP*GP*TP*TP*CP*AP*TP*TP*CP*AP*TP*AP*CP*AP*CP*T)-3') Chains: D, H
Molecule details ›
Chains: D, H
Length: 16 nucleotides
Theoretical weight: 4.81 KDa
Source organism: Arabidopsis thaliana
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 55.74Å b: 54.34Å c: 75.56Å
α: 90° β: 107.54° γ: 90°
R-values:
R R work R free
0.189 0.187 0.227
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

Meristem Initiation and de novo Stem Cell Formation.
Nicolas et al. (2022)
3 more

1 mention without citation

Structure of the unique tetrameric STENOFOLIA homeodomain bound with target promoter DNA.
Pathak et al. (2021)