› 6rz3

X-ray diffraction
4.23Å resolution

Crystal structure of a complex between the DNA-binding domain of p53 and the carboxyl-terminal conserved region of iASPP

Released:
DOI: 10.2210/pdb6rz3/pdb
PDB entry 6rz3 coloured by chain, front view.
PDB entry 6rz3 coloured by chain, side view.
PDB entry 6rz3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
iASPP mediates p53 selectivity through a modular mechanism fine-tuning DNA recognition.
Chen S, Wu J, Zhong S, Li Y, Zhang P, Ma J, Ren J, Tan Y, Wang Y, Au KF, Siebold C, Bond GL, Chen Z, Lu M, Jones EY, Lu X
Proc Natl Acad Sci U S A 116 17470-17479 (2019)
PMID: 31395738

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138122 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cellular tumor antigen p53 Chain: A
Molecule details ›
Chain: A
Length: 232 amino acids
Theoretical weight: 25.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04637 (Residues: 62-292; Coverage: 59%)
Gene names: P53, TP53
Sequence domains: P53 DNA-binding domain
RelA-associated inhibitor Chain: B
Molecule details ›
Chain: B
Length: 238 amino acids
Theoretical weight: 26.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8WUF5 (Residues: 625-828; Coverage: 25%)
Gene names: IASPP, NKIP1, PPP1R13BL, PPP1R13L, RAI
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P41212
Unit cell:
a: 71.09Å b: 71.09Å c: 255.589Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.243 0.292
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

The Role of p53 Signaling in Colorectal Cancer.
Liebl et al. (2021)
2 more

4 mentions without citation

iASPP mediates p53 selectivity through a modular mechanism fine-tuning DNA recognition.
Chen et al. (2019)
3 more