› 6rzp

X-ray diffraction
2.2Å resolution

Multicrystal structure of Proteinase K at room temperature using a multilayer monochromator.

Released:
DOI: 10.2210/pdb6rzp/pdb
PDB entry 6rzp coloured by chain, front view.
PDB entry 6rzp coloured by chain, side view.
PDB entry 6rzp coloured by chain, top view.
Source organism: Parengyodontium album
Primary publication:
Protein-to-structure pipeline for ambient-temperature crystallography at VMXi
Mikolajek H, Sanchez-Weatherby J, Sandy J, Gildea RG, Campeotto I, Cheruvara H, Clarke JD, Foster T, Fujii S, Paulsen IT, Shah BS, Hough MA
IUCrJ (2023)

Function and Biology Details

Reaction catalysed: 
Hydrolysis of keratin, and of other proteins with subtilisin-like specificity. Hydrolyzes peptide amides.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139275 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proteinase K Chain: A
Molecule details ›
Chain: A
Length: 279 amino acids
Theoretical weight: 28.96 KDa
Source organism: Parengyodontium album
Expression system: Parengyodontium album
UniProt:
  • Canonical: P06873 (Residues: 106-384; Coverage: 76%)
Gene name: PROK
Sequence domains: Subtilase family

Ligands and Environments

5 bound ligands:
Ligand GOL 1 x GOL
Ligand SO4 2 x SO4
Ligand CA 1 x CA
Ligand CL 1 x CL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE VMXi
Spacegroup: P43212
Unit cell:
a: 68.368Å b: 68.368Å c: 103.978Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.111 0.106 0.161
Expression system: Parengyodontium album

Quick links