› 6s06

X-ray diffraction
1.15Å resolution

Crystal structure of haloalkane dehalogenase LinB D147C+L177C mutant (LinB73) from Sphingobium japonicum UT26

Released:
DOI: 10.2210/pdb6s06/pdb
PDB entry 6s06 coloured by chain, front view.
PDB entry 6s06 coloured by chain, side view.
PDB entry 6s06 coloured by chain, top view.
Source organism: Sphingobium japonicum UT26S
Primary publication:
Description of Transport Tunnel in Haloalkane Dehalogenase Variant LinB D147C+L177C from Sphingobium japonicum
Iermak I, Degtjarik O, Havlickova P, Kuty M, Chaloupkova R, Damborsky J, Prudnikova T, Kuta Smatanova I
Catalysts (2021)

Function and Biology Details

Reaction catalysed: 
1-haloalkane + H(2)O = a primary alcohol + halide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112655 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Haloalkane dehalogenase Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 33.95 KDa
Source organism: Sphingobium japonicum UT26S
Expression system: Escherichia coli
UniProt:
  • Canonical: D4Z2G1 (Residues: 1-296; Coverage: 100%)
Gene names: SJA_C1-19590, linB
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 44.724Å b: 68.709Å c: 80.614Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.176 0.23
Expression system: Escherichia coli

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