› 6s0q

X-ray diffraction
2.65Å resolution

Structure of the A2A adenosine receptor determined at SwissFEL using native-SAD at 4.57 keV from 50,000 diffraction patterns

Released:
DOI: 10.2210/pdb6s0q/pdb
PDB entry 6s0q coloured by chain, front view.
PDB entry 6s0q coloured by chain, side view.
PDB entry 6s0q coloured by chain, top view.
Source organisms:
Primary publication:
Advances in long-wavelength native phasing at X-ray free-electron lasers.
Nass K, Cheng R, Vera L, Mozzanica A, Redford S, Ozerov D, Basu S, James D, Knopp G, Cirelli C, Martiel I, Casadei C, Weinert T, Nogly P, Skopintsev P, Usov I, Leonarski F, Geng T, Rappas M, Doré AS, Cooke R, Nasrollahi Shirazi S, Dworkowski F, Sharpe M, Olieric N, Bacellar C, Bohinc R, Steinmetz MO, Schertler G, Abela R, Patthey L, Schmitt B, Hennig M, Standfuss J, Wang M, Milne CJ
IUCrJ 7 965-975 (2020)
PMID: 33209311

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142061 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosine receptor A2a; Soluble cytochrome b562 Chain: A
Molecule details ›
Chain: A
Length: 433 amino acids
Theoretical weight: 48 KDa
Source organisms: Expression system: Trichoplusia ni
UniProt:
  • Canonical: P29274 (Residues: 2-208, 219-317; Coverage: 74%)
  • Canonical: P0ABE7 (Residues: 23-127; Coverage: 99%)
Gene names: ADORA2, ADORA2A, cybC
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand ZMA 1 x ZMA
Ligand OLA 7 x OLA
Ligand OLB 2 x OLB
Ligand OLC 2 x OLC
Ligand CLR 3 x CLR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SwissFEL ARAMIS BEAMLINE ESA
Spacegroup: C2221
Unit cell:
a: 40.34Å b: 180.66Å c: 143.05Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.194 0.221
Expression system: Trichoplusia ni

Quick links

Citations

1 review citation

Discerning best practices in XFEL-based biological crystallography - standards for nonstandard experiments.
Gorel et al. (2021)
14 other articles

6 mentions without citation

International Union of Basic and Clinical Pharmacology. CXII: Adenosine Receptors: A Further Update.
IJzerman et al. (2022)
5 more