› 6s3j

X-ray diffraction
1.9Å resolution

Crystal Structure of lipase from Geobacillus stearothermophilus T6 variant E134C/F149C

Released:
DOI: 10.2210/pdb6s3j/pdb
PDB entry 6s3j coloured by chain, front view.
PDB entry 6s3j coloured by chain, side view.
PDB entry 6s3j coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Bridges to Stability: Engineering Disulfide Bonds Towards Enhanced Lipase Biodiesel Synthesis
Gihaz S, Bash Y, Rush I, Shahar A, Pazy Y, Fishman A
ChemCatChem (2019)

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188034 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
triacylglycerol lipase Chain: A
Molecule details ›
Chain: A
Length: 392 amino acids
Theoretical weight: 43.86 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q93A71 (Residues: 33-418; Coverage: 92%)

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 49.593Å b: 71.587Å c: 113.861Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.24
Expression system: Escherichia coli BL21

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