› 6s5b

X-ray diffraction
3.05Å resolution

Non-square conformation of KtrA R16K mutant ring with bound ADP

Released:
DOI: 10.2210/pdb6s5b/pdb
PDB entry 6s5b coloured by chain, front view.
PDB entry 6s5b coloured by chain, side view.
PDB entry 6s5b coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Activation of a nucleotide-dependent RCK domain requires binding of a cation cofactor to a conserved site.
Teixeira-Duarte CM, Fonseca F, Morais-Cabral JH
Elife 8 (2019)
PMID: 31868587

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ktr system potassium uptake protein A Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 222 amino acids
Theoretical weight: 24.89 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: O32080 (Residues: 1-222; Coverage: 100%)
Gene names: BSU31090, ktrA, yuaA
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ADP 8 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P212121
Unit cell:
a: 85.818Å b: 137.429Å c: 202.617Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.209 0.243
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Molecular Mechanisms for Bacterial Potassium Homeostasis.
Stautz et al. (2021)
2 other articles

1 mention without citation

Activation of a nucleotide-dependent RCK domain requires binding of a cation cofactor to a conserved site.
Teixeira-Duarte et al. (2019)