› 6s6e

X-ray diffraction
2Å resolution

Crystal structure of the engineered ancestor of haloalkane dehalogenases and Renilla luciferase (AncHLD-RLuc I161_F162PinsL)

Released:
DOI: 10.2210/pdb6s6e/pdb
PDB entry 6s6e coloured by chain, front view.
PDB entry 6s6e coloured by chain, side view.
PDB entry 6s6e coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Engineering the protein dynamics of an ancestral luciferase.
Schenkmayerova A, Pinto GP, Toul M, Marek M, Hernychova L, Planas-Iglesias J, Daniel Liskova V, Pluskal D, Vasina M, Emond S, Dörr M, Chaloupkova R, Bednar D, Prokop Z, Hollfelder F, Bornscheuer UT, Damborsky J
Nat Commun 12 3616 (2021)
PMID: 34127663

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167440 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Engineered ancestor of haloalkane dehalogenases and Renilla luciferase (AncHLD-RLuc I161_F162PinsL) Chains: A, B
Molecule details ›
Chains: A, B
Length: 298 amino acids
Theoretical weight: 34.21 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 44.714Å b: 84.169Å c: 160.215Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.17 0.214
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Correlated Motions in Structural Biology.
Xu et al. (2021)
3 more

1 mention without citation

Engineering the protein dynamics of an ancestral luciferase.
Schenkmayerova et al. (2021)