› 6s6h

X-ray diffraction
2.4Å resolution

Crystal structure of the DNA binding domain of the chromosome-partitioning protein ParB complexed to the centromeric parS site

Released:
DOI: 10.2210/pdb6s6h/pdb
PDB entry 6s6h coloured by chain, front view.
PDB entry 6s6h coloured by chain, side view.
PDB entry 6s6h coloured by chain, top view.
Source organisms:
Primary publication:
Diversification of DNA-Binding Specificity by Permissive and Specificity-Switching Mutations in the ParB/Noc Protein Family.
Jalal ASB, Tran NT, Stevenson CE, Chan EW, Lo R, Tan X, Noy A, Lawson DM, Le TBK
Cell Rep 32 107928 (2020)
PMID: 32698006

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110718 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Chromosome-partitioning protein ParB Chains: A, B
Molecule details ›
Chains: A, B
Length: 143 amino acids
Theoretical weight: 15.5 KDa
Source organism: Caulobacter vibrioides NA1000
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B8GW30 (Residues: 126-254; Coverage: 42%)
Gene names: CCNA_03868, parB
Sequence domains: HTH domain found in ParB protein
DNA (5'-D(*GP*AP*TP*GP*TP*TP*TP*CP*AP*CP*GP*TP*GP*AP*AP*AP*CP*AP*TP*C)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 20 nucleotides
Theoretical weight: 6.13 KDa
Source organism: Caulobacter vibrioides
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2
Unit cell:
a: 127.099Å b: 40.717Å c: 94.004Å
α: 90° β: 121.4° γ: 90°
R-values:
R R work R free
0.217 0.216 0.232
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

2 review citations

Nucleoid-associated proteins shape chromatin structure and transcriptional regulation across the bacterial kingdom.
Amemiya et al. (2021)
1 more

1 mention without citation

Diversification of DNA-Binding Specificity by Permissive and Specificity-Switching Mutations in the ParB/Noc Protein Family.
Jalal et al. (2020)