› 6s97

X-ray diffraction
1.95Å resolution

Fragment transplantation onto a hyperstable ancestor of haloalkane dehalogenases and Renilla luciferase (Anc-FT)

Released:
DOI: 10.2210/pdb6s97/pdb
PDB entry 6s97 coloured by chain, front view.
PDB entry 6s97 coloured by chain, side view.
PDB entry 6s97 coloured by chain, top view.
Source organism: Renilla reniformis
Primary publication:
Engineering the protein dynamics of an ancestral luciferase.
Schenkmayerova A, Pinto GP, Toul M, Marek M, Hernychova L, Planas-Iglesias J, Daniel Liskova V, Pluskal D, Vasina M, Emond S, Dörr M, Chaloupkova R, Bednar D, Prokop Z, Hollfelder F, Bornscheuer UT, Damborsky J
Nat Commun 12 3616 (2021)
PMID: 34127663

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167444 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fragment transplantation onto hyperstable ancestor of haloalkane dehalogenases and Renilla luciferase (Anc-FT) Chain: A
Molecule details ›
Chain: A
Length: 294 amino acids
Theoretical weight: 33.88 KDa
Source organism: Renilla reniformis
Expression system: Escherichia coli #1/H766

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P3121
Unit cell:
a: 87.556Å b: 87.556Å c: 102.125Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.293 0.291 0.33
Expression system: Escherichia coli #1/H766

Quick links

Citations

4 review citations

Correlated Motions in Structural Biology.
Xu et al. (2021)
3 more

2 mentions without citation

Engineering the protein dynamics of an ancestral luciferase.
Schenkmayerova et al. (2021)
1 more