› 6s9a

X-ray diffraction
1.86Å resolution

Artificial GTPase-BSE dimer of human Dynamin1

Released:
DOI: 10.2210/pdb6s9a/pdb
PDB entry 6s9a coloured by chain, front view.
PDB entry 6s9a coloured by chain, side view.
PDB entry 6s9a coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Quantification and demonstration of the collective constriction-by-ratchet mechanism in the dynamin molecular motor.
Ganichkin OM, Vancraenenbroeck R, Rosenblum G, Hofmann H, Mikhailov AS, Daumke O, Noel JK
Proc Natl Acad Sci U S A 118 (2021)
PMID: 34244431

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170158 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dynamin-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 354 amino acids
Theoretical weight: 39.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q05193 (Residues: 6-746; Coverage: 41%)
Gene names: DNM, DNM1
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 4 x ZN
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 70.693Å b: 69.546Å c: 74.277Å
α: 90° β: 113.15° γ: 90°
R-values:
R R work R free
0.219 0.219 0.254
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Centronuclear Myopathy Caused by Defective Membrane Remodelling of Dynamin 2 and BIN1 Variants.
Fujise et al. (2022)
1 more