› 6sdm

X-ray diffraction
2.85Å resolution

NADH-dependent variant of TBADH

Released:
DOI: 10.2210/pdb6sdm/pdb
Model geometry
Fit model/data
PDB entry 6sdm coloured by chain, front view.
PDB entry 6sdm coloured by chain, side view.
PDB entry 6sdm coloured by chain, top view.
Source organism: Thermoanaerobacter brockii
Primary publication:
Versatile selective evolutionary pressure using synthetic defect in universal metabolism.
Sellés Vidal L, Murray JW, Heap JT
OpenAccess logo Nat Commun 12 6859 (2021)
PMID: 34824282

Function and Biology Details

Reaction catalysed: 
propan-2-ol + NADP(+) = acetone + NADPH + H(+).
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147162 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADP-dependent isopropanol dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 354 amino acids
Theoretical weight: 37.91 KDa
Source organism: Thermoanaerobacter brockii
Expression system: Escherichia coli
UniProt:
  • Canonical: P14941 (Residues: 1-352; Coverage: 100%)
Gene name: adh
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 79.123Å b: 123.946Å c: 169.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.174 0.238
Expression system: Escherichia coli

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Citations

3 review citations

A primer to directed evolution: current methodologies and future directions.
Sellés Vidal et al. (2023)
2 more