› 6sdw

Solution NMR

Solution structure of Staufen1 dsRBD3+4 - hARF1 SBS dsRNA complex.

Released:
DOI: 10.2210/pdb6sdw/pdb
PDB entry 6sdw coloured by chain, ensemble of 20 models, front view.
PDB entry 6sdw coloured by chain, ensemble of 20 models, side view.
PDB entry 6sdw coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Staufen1 reads out structure and sequence features in ARF1 dsRNA for target recognition.
Yadav DK, Zigáčková D, Zlobina M, Klumpler T, Beaumont C, Kubíčková M, Vaňáčová Š, Lukavsky PJ
Nucleic Acids Res 48 2091-2106 (2020)
PMID: 31875226

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132050 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Double-stranded RNA-binding protein Staufen homolog 1 Chain: A
Molecule details ›
Chain: A
Length: 177 amino acids
Theoretical weight: 19.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O95793 (Residues: 183-355; Coverage: 30%)
Gene names: STAU, STAU1
Sequence domains: Double-stranded RNA binding motif
hARF1 SBS dsRNA Chain: B
Molecule details ›
Chain: B
Length: 34 nucleotides
Theoretical weight: 10.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli DH5[alpha]

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 84%
Refinement method: na
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli DH5[alpha]

Quick links

Citations

4 review citations

Endogenous Double-Stranded RNA.
Sadeq et al. (2021)
3 more