› 6sei

X-ray diffraction
2.69Å resolution

Recognition and processing of branched DNA substrates by Slx1-Slx4 nuclease

Released:
DOI: 10.2210/pdb6sei/pdb
PDB entry 6sei coloured by chain, front view.
PDB entry 6sei coloured by chain, side view.
PDB entry 6sei coloured by chain, top view.
Source organisms:
Primary publication:
Recognition and processing of branched DNA substrates by Slx1-Slx4 nuclease.
Gaur V, Ziajko W, Nirwal S, Szlachcic A, Gapińska M, Nowotny M
Nucleic Acids Res 47 11681-11690 (2019)
PMID: 31584081

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120610 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (3 distinct):
GIY-YIG domain-containing protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 324 amino acids
Theoretical weight: 35.74 KDa
Source organism: Thermothielavioides terrestris
Expression system: Escherichia coli
UniProt:
  • Canonical: G2QV68 (Residues: 1-324; Coverage: 100%)
Gene name: THITE_2107775
Sequence domains:
Structure-specific endonuclease subunit SLX4 Chains: B, D
Molecule details ›
Chains: B, D
Length: 104 amino acids
Theoretical weight: 11.83 KDa
Source organism: Thermothielavioides terrestris
Expression system: Escherichia coli
UniProt:
  • Canonical: G2RCY5 (Residues: 834-936; Coverage: 11%)
Gene names: SLX4, THITE_2120630
Sequence domains: Slx4 endonuclease
DNA (32-MER) Chain: E
Molecule details ›
Chain: E
Length: 33 nucleotides
Theoretical weight: 10.13 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ZN 4 x ZN
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 60.075Å b: 92.7Å c: 93.251Å
α: 90° β: 91.603° γ: 90°
R-values:
R R work R free
0.215 0.208 0.266
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Coordinated roles of SLX4 and MutSβ in DNA repair and the maintenance of genome stability.
Young et al. (2021)
2 more

5 mentions without citation

Structural Aspects of DNA Repair and Recombination in Crop Improvement.
Verma et al. (2020)
4 more