› 6sgz

Electron Microscopy
3.9Å resolution

Structure of protomer 2 of the ESX-3 core complex

Released:
DOI: 10.2210/pdb6sgz/pdb
PDB entry 6sgz coloured by chain, front view.
PDB entry 6sgz coloured by chain, side view.
PDB entry 6sgz coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Primary publication:
Architecture of the mycobacterial type VII secretion system.
Famelis N, Rivera-Calzada A, Degliesposti G, Wingender M, Mietrach N, Skehel JM, Fernandez-Leiro R, Böttcher B, Schlosser A, Llorca O, Geibel S
Nature 576 321-325 (2019)
PMID: 31597161
Related structures: EMD-10191

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-106242 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
ESX-3 secretion system protein EccD3 Chains: E, H
Molecule details ›
Chains: E, H
Length: 467 amino acids
Theoretical weight: 47.34 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QQ46 (Residues: 6-472; Coverage: 98%)
Gene names: MSMEG_0623, MSMEI_0607, eccD3, snm
Sequence domains: WXG100 protein secretion system (Wss), protein YukD
ESX-3 secretion system ATPase EccB3 Chain: I
Molecule details ›
Chain: I
Length: 59 amino acids
Theoretical weight: 6.58 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QQ39 (Residues: 33-91; Coverage: 11%)
Gene names: MSMEG_0616, MSMEI_0600, eccB3
ESX-3 secretion system protein EccC3 Chain: J
Molecule details ›
Chain: J
Length: 343 amino acids
Theoretical weight: 38.3 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Mycolicibacterium smegmatis MC2 155
ESX-3 secretion system protein EccE3 Chain: D
Molecule details ›
Chain: D
Length: 286 amino acids
Theoretical weight: 30.85 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QQ48 (Residues: 2-287; Coverage: 93%)
Gene names: MSMEG_0626, MSMEI_0609, eccE3
Sequence domains: Putative type VII ESX secretion system translocon, EccE

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-10191
Expression system: Mycolicibacterium smegmatis MC2 155

Quick links

Citations

9 review citations

Transporters Involved in the Biogenesis and Functionalization of the Mycobacterial Cell Envelope.
Jackson et al. (2021)
8 more

2 mentions without citation

Recent Insights into the Structure and Function of Mycobacterial Membrane Proteins Facilitated by Cryo-EM.
Bendre et al. (2021)
1 more