› 6shc

X-ray diffraction
3.55Å resolution

Crystal structure of human IRE1 luminal domain Q105C

Released:
DOI: 10.2210/pdb6shc/pdb
PDB entry 6shc coloured by chain, front view.
PDB entry 6shc coloured by chain, side view.
PDB entry 6shc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Unstructured regions in IRE1α specify BiP-mediated destabilisation of the luminal domain dimer and repression of the UPR.
Amin-Wetzel N, Neidhardt L, Yan Y, Mayer MP, Ron D
Elife 8 (2019)
PMID: 31873072

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130962 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase/endoribonuclease IRE1 Chain: A
Molecule details ›
Chain: A
Length: 367 amino acids
Theoretical weight: 41.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O75460 (Residues: 24-390; Coverage: 38%)
Gene names: ERN1, IRE1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P6522
Unit cell:
a: 182.772Å b: 182.772Å c: 68.449Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.324 0.323 0.332
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Mechanisms, regulation and functions of the unfolded protein response.
Hetz et al. (2020)
8 more

2 mentions without citation

HEPN RNases - an emerging class of functionally distinct RNA processing and degradation enzymes.
Pillon et al. (2021)
1 more