› 6sjj

X-ray diffraction
2.3Å resolution

A new modulated crystal structure of ANS complex of St John's wort Hyp-1 protein with 36 protein molecules in the asymmetric unit of the supercell

Released:
DOI: 10.2210/pdb6sjj/pdb
PDB entry 6sjj coloured by chain, front view.
PDB entry 6sjj coloured by chain, side view.
PDB entry 6sjj coloured by chain, top view.
Source organism: Hypericum perforatum
Primary publication:
A new modulated crystal structure of the ANS complex of the St John's wort Hyp-1 protein with 36 protein molecules in the asymmetric unit of the supercell.
Smietanska J, Sliwiak J, Gilski M, Dauter Z, Strzalka R, Wolny J, Jaskolski M
Acta Crystallogr D Struct Biol 76 653-667 (2020)
PMID: 32627738

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo 36-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103051 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bet v I/Major latex protein domain-containing protein Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z, a, b, c, d, e, f, g, h, i, j
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z, a, b, c, d, e, f, g, h, i, j
Length: 163 amino acids
Theoretical weight: 18.32 KDa
Source organism: Hypericum perforatum
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A160HRF0 (Residues: 1-159; Coverage: 100%)
Gene name: PR10.1
Sequence domains: Pathogenesis-related protein Bet v 1 family

Ligands and Environments

5 bound ligands:
Ligand 2AN 157 x 2AN
Ligand SO4 10 x SO4
Ligand EPE 1 x EPE
Ligand DMS 3 x DMS
Ligand FLC 7 x FLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: C2
Unit cell:
a: 145.85Å b: 145.85Å c: 385.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.255
Expression system: Escherichia coli

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Citations

2 mentions without citation

A new modulated crystal structure of the ANS complex of the St John's wort Hyp-1 protein with 36 protein molecules in the asymmetric unit of the supercell.
Smietanska et al. (2020)
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