› 6sl7

X-ray diffraction
3.3Å resolution

The Delta Calcium mutant of ALPHA-ACTININ FROM ENTAMOEBA HISTOLYTICA

Released:
DOI: 10.2210/pdb6sl7/pdb
PDB entry 6sl7 coloured by chain, front view.
PDB entry 6sl7 coloured by chain, side view.
PDB entry 6sl7 coloured by chain, top view.
Source organism: Entamoeba histolytica
Primary publication:
Calcium modulates the domain flexibility and function of an α-actinin similar to the ancestral α-actinin.
Pinotsis N, Zielinska K, Babuta M, Arolas JL, Kostan J, Khan MB, Schreiner C, Salmazo A, Ciccarelli L, Puchinger M, Gkougkoulia EA, Ribeiro EA, Marlovits TC, Bhattacharya A, Djinovic-Carugo K
Proc Natl Acad Sci U S A 117 22101-22112 (2020)
PMID: 32848067

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111312 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calponin homology domain protein, putative Chain: A
Molecule details ›
Chain: A
Length: 621 amino acids
Theoretical weight: 69.85 KDa
Source organism: Entamoeba histolytica
Expression system: Escherichia coli
UniProt:
  • Canonical: C4LWU6 (Residues: 1-619; Coverage: 100%)
Gene name: EHI_199000
Sequence domains: Calponin homology (CH) domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MLZ 1 x MLZ

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P2221
Unit cell:
a: 40.739Å b: 75.526Å c: 234.252Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.234 0.308
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Actin Bundles Dynamics and Architecture.
Rajan et al. (2023)
1 more