› 6slo

X-ray diffraction
1.94Å resolution

Crystal structure of PUF60 UHM domain in complex with 7,8 dimethoxyperphenazine

Released:
DOI: 10.2210/pdb6slo/pdb
PDB entry 6slo coloured by chain, front view.
PDB entry 6slo coloured by chain, side view.
PDB entry 6slo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Identification of phenothiazine derivatives as UHM-binding inhibitors of early spliceosome assembly.
Jagtap PKA, Kubelka T, Soni K, Will CL, Garg D, Sippel C, Kapp TG, Potukuchi HK, Schorpp K, Hadian K, Kessler H, Lührmann R, Hausch F, Bach T, Sattler M
Nat Commun 11 5621 (2020)
PMID: 33159082

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193769 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly(U)-binding-splicing factor PUF60 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 222 amino acids
Theoretical weight: 24.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UHX1 (Residues: 460-559; Coverage: 18%)
Gene names: FIR, PUF60, ROBPI, SIAHBP1

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand LJT 2 x LJT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21212
Unit cell:
a: 81.52Å b: 89.4Å c: 149.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.207
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Dysregulation and therapeutic targeting of RNA splicing in cancer.
Stanley et al. (2022)
6 more