› 6sm0

X-ray diffraction
1.91Å resolution

Venus 66 p-Azido-L-Phenylalanin (azF) variant, dark grown

Released:
DOI: 10.2210/pdb6sm0/pdb
PDB entry 6sm0 coloured by chain, front view.
PDB entry 6sm0 coloured by chain, side view.
PDB entry 6sm0 coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Stalling chromophore synthesis of the fluorescent protein Venus reveals the molecular basis of the final oxidation step.
Auhim HS, Grigorenko BL, Harris TK, Aksakal OE, Polyakov IV, Berry C, Gomes GDP, Alabugin IV, Rizkallah PJ, Nemukhin AV, Jones DD
Chem Sci 12 7735-7745 (2021)
PMID: 34168826

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 227 amino acids
Theoretical weight: 25.69 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 2-230; Coverage: 96%)
Gene name: GFP
Sequence domains: Green fluorescent protein

Ligands and Environments

3 bound ligands:
Ligand OXY 1 x OXY
Ligand ZN 1 x ZN
Ligand SO4 1 x SO4
1 modified residue:
Ligand LKE 1 x LKE

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 50.947Å b: 63.251Å c: 69.915Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.195 0.243
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Fluorescent Proteins: Crystallization, Structural Determination, and Nonnatural Amino Acid Incorporation.
Ahmed et al. (2023)

1 mention without citation

Stalling chromophore synthesis of the fluorescent protein Venus reveals the molecular basis of the final oxidation step.
Auhim et al. (2021)