› 6so5

Electron Microscopy
4.2Å resolution

Homo sapiens WRB/CAML heterotetramer in complex with a TRC40 dimer

Released:
DOI: 10.2210/pdb6so5/pdb
PDB entry 6so5 coloured by chain, front view.
PDB entry 6so5 coloured by chain, side view.
PDB entry 6so5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Basis of Tail-Anchored Membrane Protein Biogenesis by the GET Insertase Complex.
McDowell MA, Heimes M, Fiorentino F, Mehmood S, Farkas Á, Coy-Vergara J, Wu D, Bolla JR, Schmid V, Heinze R, Wild K, Flemming D, Pfeffer S, Schwappach B, Robinson CV, Sinning I
Mol Cell 80 72-86.e7 (2020)
PMID: 32910895
Related structures: EMD-10266

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126209 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ATPase GET3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 360 amino acids
Theoretical weight: 40.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43681 (Residues: 1-348; Coverage: 100%)
Gene names: ARSA, ASNA1, GET3, TRC40
Sequence domains: Anion-transporting ATPase
Guided entry of tail-anchored proteins factor 1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 184 amino acids
Theoretical weight: 20.82 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O00258 (Residues: 1-174; Coverage: 100%)
Gene names: CHD5, GET1, WRB
Sequence domains: CHD5-like protein
Guided entry of tail-anchored proteins factor CAMLG Chains: E, F
Molecule details ›
Chains: E, F
Length: 127 amino acids
Theoretical weight: 14.28 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P49069 (Residues: 185-296; Coverage: 38%)
Gene names: CAML, CAMLG, GET2
Sequence domains: Get2-like

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-10266
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda

Quick links

Citations

14 review citations

Structural and molecular mechanisms for membrane protein biogenesis by the Oxa1 superfamily.
McDowell et al. (2021)
13 more

2 mentions without citation

Translocation of Proteins through a Distorted Lipid Bilayer.
Wu et al. (2021)
1 more