› 6spz

X-ray diffraction
2.08Å resolution

Crystal structure of PDZ1-2 from PSD-95 with peptide ligand sequence RRESEI bound to both domains

Released:
DOI: 10.2210/pdb6spz/pdb
PDB entry 6spz coloured by chain, front view.
PDB entry 6spz coloured by chain, side view.
PDB entry 6spz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The Dual PDZ Domain from Postsynaptic Density Protein 95 Forms a Scaffold with Peptide Ligand.
Rodzli NA, Lockhart-Cairns MP, Levy CW, Chipperfield J, Bird L, Baldock C, Prince SM
Biophys J 119 667-689 (2020)
PMID: 32652058

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159064 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Disks large homolog 4 Chain: A
Molecule details ›
Chain: A
Length: 197 amino acids
Theoretical weight: 20.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P78352 (Residues: 55-249; Coverage: 27%)
Gene names: DLG4, PSD95
Sequence domains: PDZ domain
Inward rectifier potassium channel 2 Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 6 amino acids
Theoretical weight: 791 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P63252 (Residues: 422-427; Coverage: 1%)
Gene names: IRK1, KCNJ2

Ligands and Environments

1 bound ligand:
Ligand GSH 1 x GSH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: I41
Unit cell:
a: 50.5Å b: 50.5Å c: 176.37Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.2 0.24
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

2 citation in other articles

Bidirectional protein-protein interactions control liquid-liquid phase separation of PSD-95 and its interaction partners.
Christensen et al. (2022)
1 more

3 mentions without citation

The Dual PDZ Domain from Postsynaptic Density Protein 95 Forms a Scaffold with Peptide Ligand.
Rodzli et al. (2020)
2 more