› 6sq2

X-ray diffraction
1.68Å resolution

Structure of a phosphomimetic switch 2 variant of Rab8a in complex with the phospho-Rab binding domain of RILPL2

Released:
DOI: 10.2210/pdb6sq2/pdb
PDB entry 6sq2 coloured by chain, front view.
PDB entry 6sq2 coloured by chain, side view.
PDB entry 6sq2 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Khan AR, Waschbusch D

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158170 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ras-related protein Rab-8A Chains: A, B
Molecule details ›
Chains: A, B
Length: 184 amino acids
Theoretical weight: 21.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61006 (Residues: 1-181; Coverage: 87%)
Gene names: MEL, RAB8, RAB8A
Sequence domains: Ras family
RILP-like protein 2 Chains: D, E
Molecule details ›
Chains: D, E
Length: 43 amino acids
Theoretical weight: 5.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q969X0 (Residues: 129-165; Coverage: 18%)
Gene names: RILPL2, RLP2
Sequence domains: Rab interacting lysosomal protein

Ligands and Environments

2 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 60.698Å b: 71.733Å c: 116.39Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.182 0.202
Expression system: Escherichia coli

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