› 6sri

Electron Microscopy
4.2Å resolution

Structure of the Fanconi anaemia core complex

Released:
DOI: 10.2210/pdb6sri/pdb
PDB entry 6sri coloured by chain, front view.
PDB entry 6sri coloured by chain, side view.
PDB entry 6sri coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Structure of the Fanconi anaemia monoubiquitin ligase complex.
Shakeel S, Rajendra E, Alcón P, O'Reilly F, Chorev DS, Maslen S, Degliesposti G, Russo CJ, He S, Hill CH, Skehel JM, Scheres SHW, Patel KJ, Rappsilber J, Robinson CV, Passmore LA
Nature 575 234-237 (2019)
PMID: 31666700
Related structures: EMD-10290

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 47-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167473 (preferred)
Entry contents:
27 distinct polypeptide molecules
Macromolecules (27 distinct):
Unassigned secondary structure elements (central region, proposed FANCB-FAAP100: chain A,a; base region, proposed FANCC-FANC-E-FANCF: chain 3) Chains: 3, A, a
Molecule details ›
Chains: 3, A, a
Length: 76 amino acids
Theoretical weight: 6.49 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
Unassigned secondary structure elements (central region, proposed FANCB-FAAP100) Chains: B, b
Molecule details ›
Chains: B, b
Length: 25 amino acids
Theoretical weight: 2.15 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
Unassigned secondary structure elements (central region, proposed FANCB-FAAP100) Chains: C, c
Molecule details ›
Chains: C, c
Length: 30 amino acids
Theoretical weight: 2.57 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
Unassigned secondary structure elements (central region, proposed FANCB-FAAP100) Chains: D, d
Molecule details ›
Chains: D, d
Length: 23 amino acids
Theoretical weight: 1.98 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
Unassigned secondary structure elements (central region, proposed FANCB-FAAP100) Chains: E, e
Molecule details ›
Chains: E, e
Length: 9 amino acids
Theoretical weight: 784 Da
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
Unassigned secondary structure elements (central region, proposed FANCB-FAAP100: chain F,f; base region, proposed FANCC-FANC-E-FANCF: chain Z) Chains: F, Z, f
Molecule details ›
Chains: F, Z, f
Length: 15 amino acids
Theoretical weight: 1.29 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
Unassigned secondary structure elements (central region, proposed FANCB-FAAP100) Chains: G, g
Molecule details ›
Chains: G, g
Length: 26 amino acids
Theoretical weight: 2.23 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
Unassigned secondary structure elements (central region, proposed FANCB-FAAP100) Chains: H, J, h, j
Molecule details ›
Chains: H, J, h, j
Length: 17 amino acids
Theoretical weight: 1.46 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
Unassigned secondary structure elements (central region, proposed FANCB-FAAP100) Chains: I, i
Molecule details ›
Chains: I, i
Length: 37 amino acids
Theoretical weight: 3.17 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
Unassigned secondary structure elements (central region, proposed FANCB-FAAP100) Chains: K, k
Molecule details ›
Chains: K, k
Length: 21 amino acids
Theoretical weight: 1.81 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
Unassigned secondary structure elements (central region, proposed FANCB-FAAP100) Chains: L, l
Molecule details ›
Chains: L, l
Length: 18 amino acids
Theoretical weight: 1.55 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
Unassigned secondary structure elements (proposed FANCB) Chains: M, m
Molecule details ›
Chains: M, m
Length: 120 amino acids
Theoretical weight: 10.23 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
Unassigned secondary structure elements (proposed FAAP100) Chains: N, n
Molecule details ›
Chains: N, n
Length: 43 amino acids
Theoretical weight: 3.68 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
Unassigned secondary structure elements (proposed FANCB) Chains: O, o
Molecule details ›
Chains: O, o
Length: 279 amino acids
Theoretical weight: 23.76 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
Unassigned secondary structure elements (proposed FAAP100) Chains: P, p
Molecule details ›
Chains: P, p
Length: 276 amino acids
Theoretical weight: 23.51 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
Unassigned secondary structure elements (base region, proposed FANCC-FANC-E-FANCF) Chain: U
Molecule details ›
Chain: U
Length: 227 amino acids
Theoretical weight: 19.34 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
Unassigned secondary structure elements (base region, proposed FANCC-FANC-E-FANCF) Chain: V
Molecule details ›
Chain: V
Length: 27 amino acids
Theoretical weight: 2.32 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
Unassigned secondary structure elements (base region, proposed FANCC-FANC-E-FANCF) Chain: W
Molecule details ›
Chain: W
Length: 20 amino acids
Theoretical weight: 1.72 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
Unassigned secondary structure elements (base region, proposed FANCC-FANC-E-FANCF) Chain: X
Molecule details ›
Chain: X
Length: 13 amino acids
Theoretical weight: 1.12 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
Unassigned secondary structure elements (base region, proposed FANCC-FANC-E-FANCF) Chain: Y
Molecule details ›
Chain: Y
Length: 84 amino acids
Theoretical weight: 7.17 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
Unassigned secondary structure elements (base region, proposed FANCC-FANC-E-FANCF) Chain: 1
Molecule details ›
Chain: 1
Length: 109 amino acids
Theoretical weight: 9.29 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
Unassigned secondary structure elements (base region, proposed FANCC-FANC-E-FANCF) Chain: 2
Molecule details ›
Chain: 2
Length: 62 amino acids
Theoretical weight: 5.29 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
Unassigned secondary structure elements (base region, proposed FANCC-FANC-E-FANCF) Chain: 4
Molecule details ›
Chain: 4
Length: 71 amino acids
Theoretical weight: 6.06 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
Fanconi anemia protein FANCL Chains: Q, q
Molecule details ›
Chains: Q, q
Length: 373 amino acids
Theoretical weight: 42.69 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q3MUH5 (Residues: 1-373; Coverage: 100%)
Gene name: FANCL
Sequence domains:
base region, proposed FANCF Chain: T
Molecule details ›
Chain: T
Length: 201 amino acids
Theoretical weight: 17.12 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
Unassigned secondary structure elements (top region, proposed FANCG) Chain: R
Molecule details ›
Chain: R
Length: 285 amino acids
Theoretical weight: 24.27 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
Unassigned secondary structure elements (top region, proposed FANCG) Chain: S
Molecule details ›
Chain: S
Length: 145 amino acids
Theoretical weight: 12.36 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-10290
Expression system: Spodoptera frugiperda

Quick links

Citations

19 review citations

Fanconi anemia and the underlying causes of genomic instability.
Rageul et al. (2020)
18 more

5 mentions without citation

Structural insight into FANCI-FANCD2 monoubiquitination.
Li et al. (2020)
4 more