› 6ssp

X-ray diffraction
3.25Å resolution

Structure of the pentameric ligand-gated ion channel ELIC in complex with a NAM nanobody

Released:
DOI: 10.2210/pdb6ssp/pdb
Model geometry
Fit model/data
PDB entry 6ssp coloured by chain, front view.
PDB entry 6ssp coloured by chain, side view.
PDB entry 6ssp coloured by chain, top view.
Source organisms:
Primary publication:
Modulation of the Erwinia ligand-gated ion channel (ELIC) and the 5-HT3 receptor via a common vestibule site.
Brams M, Govaerts C, Kambara K, Price KL, Spurny R, Gharpure A, Pardon E, Evans GL, Bertrand D, Lummis SC, Hibbs RE, Steyaert J, Ulens C
OpenAccess logo Elife 9 (2020)
PMID: 31990273

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209606 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cys-loop ligand-gated ion channel Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 318 amino acids
Theoretical weight: 36.47 KDa
Source organism: Dickeya chrysanthemi
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C7B7 (Residues: 8-163, 164-320; Coverage: 98%)
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
NANOBODY 21 Chains: K, L
Molecule details ›
Chains: K, L
Length: 139 amino acids
Theoretical weight: 15.25 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand CA 8 x CA
Ligand UMQ 10 x UMQ
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P1
Unit cell:
a: 121.804Å b: 122.354Å c: 128.221Å
α: 71.02° β: 64.19° γ: 61.66°
R-values:
R R work R free
0.249 0.248 0.264
Expression system: Escherichia coli

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Citations

8 citation in other articles

Modulation of the Erwinia ligand-gated ion channel (ELIC) and the 5-HT3 receptor via a common vestibule site.
Brams et al. (2020)
7 more

2 mentions without citation

VHH Nanobody Versatility against Pentameric Ligand-Gated Ion Channels.
Nemecz et al. (2024)
1 more