› 6sw5

X-ray diffraction
2.35Å resolution

Crystal structure of the human S-adenosylmethionine synthetase 1 (ligand-free form)

Released:
DOI: 10.2210/pdb6sw5/pdb
PDB entry 6sw5 coloured by chain, front view.
PDB entry 6sw5 coloured by chain, side view.
PDB entry 6sw5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of the dominant inheritance of hypermethioninemia associated with the Arg264His mutation in the MAT1A gene.
Panmanee J, Antonyuk SV, Hasnain SS
Acta Crystallogr D Struct Biol 76 594-607 (2020)
PMID: 32496220

Function and Biology Details

Reaction catalysed: 
ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169403 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-adenosylmethionine synthase isoform type-1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 395 amino acids
Theoretical weight: 43.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q00266 (Residues: 1-395; Coverage: 100%)
Gene names: AMS1, MAT1A, MATA1
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand PEG 2 x PEG
Ligand EDO 12 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2
Unit cell:
a: 218.684Å b: 61Å c: 119.762Å
α: 90° β: 90.55° γ: 90°
R-values:
R R work R free
0.191 0.188 0.239
Expression system: Escherichia coli BL21(DE3)

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Citations

1 citations in other articles

Novel HPD mutation p.A244V compound with p.T219M causing tyrosinemia type III in a Chinese girl and review of the genotype-phenotype spectrum.
Han et al. (2023)

3 mentions without citation

Implications of divergence of methionine adenosyltransferase in archaea.
Chouhan et al. (2022)
2 more