› 6sy4

X-ray diffraction
2.7Å resolution

TetR in complex with the TetR-binding RNA-aptamer K1

Released:
DOI: 10.2210/pdb6sy4/pdb
PDB entry 6sy4 coloured by chain, front view.
PDB entry 6sy4 coloured by chain, side view.
PDB entry 6sy4 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The complex formed between a synthetic RNA aptamer and the transcription repressor TetR is a structural and functional twin of the operator DNA-TetR regulator complex.
Grau FC, Jaeger J, Groher F, Suess B, Muller YA
Nucleic Acids Res 48 3366-3378 (2020)
PMID: 32052019

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137873 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Tetracycline repressor protein class B from transposon Tn10 Chains: A, B
Molecule details ›
Chains: A, B
Length: 208 amino acids
Theoretical weight: 23.53 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04483 (Residues: 1-203; Coverage: 98%)
Gene name: tetR
Sequence domains:
TetR-binding aptamer K1 (43-MER) Chain: C
Molecule details ›
Chain: C
Length: 43 nucleotides
Theoretical weight: 14.04 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P42212
Unit cell:
a: 96.027Å b: 96.027Å c: 163.015Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.201 0.246
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

2 review citations

Status quo of tet regulation in bacteria.
Bertram et al. (2022)
1 more

5 mentions without citation

Structural Biology for the Molecular Insight between Aptamers and Target Proteins.
Zhang et al. (2021)
4 more