› 6t1f

X-ray diffraction
2.9Å resolution

Crystal structure of the C-terminally truncated chromosome-partitioning protein ParB from Caulobacter crescentus complexed to the centromeric parS site

Released:
DOI: 10.2210/pdb6t1f/pdb
PDB entry 6t1f coloured by chain, front view.
PDB entry 6t1f coloured by chain, side view.
PDB entry 6t1f coloured by chain, top view.
Source organisms:
Primary publication:
A CTP-dependent gating mechanism enables ParB spreading on DNA.
Jalal AS, Tran NT, Stevenson CE, Chimthanawala A, Badrinarayanan A, Lawson DM, Le TB
Elife 10 (2021)
PMID: 34397383

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110719 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Chromosome-partitioning protein ParB Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 257 amino acids
Theoretical weight: 28.02 KDa
Source organism: Caulobacter vibrioides NA1000
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B8GW30 (Residues: 11-254; Coverage: 80%)
Gene names: CCNA_03868, parB
Sequence domains:
DNA (5'-D(*GP*GP*AP*TP*GP*TP*TP*TP*CP*AP*CP*GP*TP*GP*AP*AP*AP*CP*AP*TP*CP*C)-3') Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 22 nucleotides
Theoretical weight: 6.75 KDa
Source organism: Caulobacter vibrioides
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P21
Unit cell:
a: 54.25Å b: 172.928Å c: 72.852Å
α: 90° β: 90.54° γ: 90°
R-values:
R R work R free
0.241 0.24 0.263
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

2 review citations

Catching a Walker in the Act-DNA Partitioning by ParA Family of Proteins.
Mishra et al. (2022)
1 more

3 mentions without citation

A CTP-dependent gating mechanism enables ParB spreading on DNA.
Jalal et al. (2021)
2 more