› 6t7s

Electron Microscopy
4.5Å resolution

MexB structure solved by cryo-EM in nanodisc in absence of its protein partners

Released:
DOI: 10.2210/pdb6t7s/pdb
PDB entry 6t7s coloured by chain, front view.
PDB entry 6t7s coloured by chain, side view.
PDB entry 6t7s coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Antibiotic export by MexB multidrug efflux transporter is allosterically controlled by a MexA-OprM chaperone-like complex.
Glavier M, Puvanendran D, Salvador D, Decossas M, Phan G, Garnier C, Frezza E, Cece Q, Schoehn G, Picard M, Taveau JC, Daury L, Broutin I, Lambert O
Nat Commun 11 4948 (2020)
PMID: 33009415
Related structures: EMD-10371

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156447 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multidrug resistance protein MexB Chains: J, K, L
Molecule details ›
Chains: J, K, L
Length: 1052 amino acids
Theoretical weight: 113.71 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P52002 (Residues: 1-1046; Coverage: 100%)
Gene names: PA0426, mexB
Sequence domains: AcrB/AcrD/AcrF family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.5Å
Relevant EMDB volumes: EMD-10371
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Ever-Adapting RND Efflux Pumps in Gram-Negative Multidrug-Resistant Pathogens: A Race against Time.
Zwama et al. (2021)
11 more

2 mentions without citation

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
Alav et al. (2021)
1 more