› 6tac

X-ray diffraction
1.6Å resolution

Human NAMPT deletion mutant in complex with nicotinamide mononucleotide, pyrophosphate, and Mg2+

Released:
DOI: 10.2210/pdb6tac/pdb
Model geometry
Fit model/data
PDB entry 6tac coloured by chain, front view.
PDB entry 6tac coloured by chain, side view.
PDB entry 6tac coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Houry D, Raasakka A, Kursula P, Ziegler M

Function and Biology Details

Reaction catalysed: 
Nicotinamide D-ribonucleotide + diphosphate = nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155082 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nicotinamide phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 493 amino acids
Theoretical weight: 55.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P43490 (Residues: 1-491; Coverage: 98%)
Gene names: NAMPT, PBEF, PBEF1
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand GOL 1 x GOL
Ligand MG 4 x MG
Ligand NMN 2 x NMN
Ligand PPV 2 x PPV
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P21
Unit cell:
a: 61.017Å b: 106.666Å c: 83.549Å
α: 90° β: 96.623° γ: 90°
R-values:
R R work R free
0.144 0.143 0.171
Expression system: Escherichia coli

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