6tbl

X-ray diffraction
2.65Å resolution

Crystal structure of MMS19(CTD)-CIAO1-CIAO2B CIA targeting complex

Released:
Primary publication:
Structural insights into Fe-S protein biogenesis by the CIA targeting complex.
Nat Struct Mol Biol 27 735-742 (2020)
PMID: 32632277

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-181532 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
MMS19 nucleotide excision repair protein homolog Chains: A, B
Molecule details ›
Chains: A, B
Length: 125 amino acids
Theoretical weight: 13.57 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9D071 (Residues: 911-1031; Coverage: 12%)
Gene names: Mms19, Mms19l
MIP18 family protein galla-2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 159 amino acids
Theoretical weight: 17.81 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9VTC4 (Residues: 2-156; Coverage: 99%)
Gene names: CG7949, galla-2
Sequence domains: Iron-sulfur cluster assembly protein
Probable cytosolic iron-sulfur protein assembly protein Ciao1 Chains: E, F
Molecule details ›
Chains: E, F
Length: 338 amino acids
Theoretical weight: 37.28 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q7K1Y4 (Residues: 2-335; Coverage: 100%)
Gene names: CG12797, Ciao1
Sequence domains: WD domain, G-beta repeat

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P4212
Unit cell:
a: 144.07Å b: 144.07Å c: 166.43Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.242
Expression system: Trichoplusia ni