› 6tce

X-ray diffraction
2.92Å resolution

Crystal structure of the GGCT site-bound MH1 domain of Smad5 containing a GGGS insertion in the Loop1

Released:
DOI: 10.2210/pdb6tce/pdb
PDB entry 6tce coloured by chain, front view.
PDB entry 6tce coloured by chain, side view.
PDB entry 6tce coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Unveiling the dimer/monomer propensities of Smad MH1-DNA complexes.
Ruiz L, Kaczmarska Z, Gomes T, Aragon E, Torner C, Freier R, Baginski B, Martin-Malpartida P, de Martin Garrido N, Marquez JA, Cordeiro TN, Pluta R, Macias MJ
Comput Struct Biotechnol J 19 632-646 (2021)
PMID: 33510867

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120645 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Mothers against decapentaplegic homolog 5 Chain: A
Molecule details ›
Chain: A
Length: 134 amino acids
Theoretical weight: 15.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99717 (Residues: 9-22, 23-138; Coverage: 28%)
Gene names: MADH5, SMAD5
Sequence domains: MH1 domain
DNA (5'-D(P*TP*GP*CP*AP*GP*GP*CP*TP*AP*GP*CP*CP*TP*GP*CP*A)-3') Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.9 KDa

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: I4122
Unit cell:
a: 106.123Å b: 106.123Å c: 82.395Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.253
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Canonical TGFβ Signaling and Its Contribution to Endometrial Cancer Development and Progression-Underestimated Target of Anticancer Strategies.
Zakrzewski PK. (2021)
2 other articles

1 mention without citation

Unveiling the dimer/monomer propensities of Smad MH1-DNA complexes.
Ruiz et al. (2021)