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› 6tcq

X-ray diffraction
2.05Å resolution

Crystal structure of the omalizumab Fab Ser81Arg and Gln83Arg light chain mutant

Released:
DOI: 10.2210/pdb6tcq/pdb
Model geometry
Fit model/data
PDB entry 6tcq coloured by chain, front view.
PDB entry 6tcq coloured by chain, side view.
PDB entry 6tcq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Engineering the Fab fragment of the anti-IgE omalizumab to prevent Fab crystallization and permit IgE-Fc complex crystallization.
Mitropoulou AN, Ceska T, Heads JT, Beavil AJ, Henry AJ, McDonnell JM, Sutton BJ, Davies AM
OpenAccess logo Acta Crystallogr F Struct Biol Commun 76 116-129 (2020)
PMID: 32133997

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-214202 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Omalizumab Fab Ser81Arg and Gln83Arg light chain mutant Chain: H
Molecule details ›
Chain: H
Length: 230 amino acids
Theoretical weight: 24.67 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Omalizumab Fab Ser81Arg and Gln83Arg light chain mutant Chain: L
Molecule details ›
Chain: L
Length: 218 amino acids
Theoretical weight: 24.02 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 44.033Å b: 96.615Å c: 103.509Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.221
Expression system: Homo sapiens

Quick links

Citations

4 citation in other articles

Hallucinating structure-conditioned antibody libraries for target-specific binders.
Mahajan et al. (2022)
3 more

2 mentions without citation

IMM-BCP-01, a patient-derived anti-SARS-CoV-2 antibody cocktail, is active across variants of concern including Omicron BA.1 and BA.2.
Nikitin et al. (2022)
1 more