› 6teq

X-ray diffraction
1.44Å resolution

Crystal structure of a galactokinase from Bifidobacterium infantis in complex with 2-deoxy-2-fluoro-galactose

Released:
DOI: 10.2210/pdb6teq/pdb
PDB entry 6teq coloured by chain, front view.
PDB entry 6teq coloured by chain, side view.
PDB entry 6teq coloured by chain, top view.
Source organism: Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM 20088
Primary publication:
Profiling Substrate Promiscuity of Wild-Type Sugar Kinases for Multi-fluorinated Monosaccharides.
Keenan T, Parmeggiani F, Malassis J, Fontenelle CQ, Vendeville JB, Offen W, Both P, Huang K, Marchesi A, Heyam A, Young C, Charnock SJ, Davies GJ, Linclau B, Flitsch SL, Fascione MA
Cell Chem Biol 27 1199-1206.e5 (2020)
PMID: 32619452

Function and Biology Details

Reaction catalysed: 
ATP + alpha-D-galactose = ADP + alpha-D-galactose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110477 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
galactokinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 429 amino acids
Theoretical weight: 45.82 KDa
Source organism: Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM 20088
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B7GUI0 (Residues: 1-416; Coverage: 100%)
Gene name: Blon_2062
Sequence domains:

Ligands and Environments

8 bound ligands:
Ligand MES 2 x MES
Ligand PEG 17 x PEG
Ligand GOL 14 x GOL
Ligand GAF 4 x GAF
Ligand 2FG 4 x 2FG
Ligand CL 7 x CL
Ligand EDO 2 x EDO
Ligand PGE 2 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 52.218Å b: 164.386Å c: 115.874Å
α: 90° β: 95.94° γ: 90°
R-values:
R R work R free
0.17 0.169 0.203
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Biocatalytic Approaches to Building Blocks for Enzymatic and Chemical Glycan Synthesis.
Dolan et al. (2023)
6 other articles