› 6thq

X-ray diffraction
2.15Å resolution

Crystal structure of branched-chain aminotransferase from thermophilic archaea Thermoproteus uzoniensis with norvaline

Released:
DOI: 10.2210/pdb6thq/pdb
PDB entry 6thq coloured by chain, front view.
PDB entry 6thq coloured by chain, side view.
PDB entry 6thq coloured by chain, top view.
Source organism: Thermoproteus uzoniensis 768-20
Primary publication:
Three-Dimensional Structure of Branched-Chain Amino Acid Transaminase from Thermoproteus uzoniensis in Complex with L-Norvaline
Boyko KM, Nikolaeva AY, Timofeev VI, Popov VO, Bezsudnova EY
Crystallography Reports (2020)

Function and Biology Details

Reaction catalysed: 
L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123383 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Branched-chain-amino-acid aminotransferase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 307 amino acids
Theoretical weight: 34.29 KDa
Source organism: Thermoproteus uzoniensis 768-20
Expression system: Escherichia coli
UniProt:
  • Canonical: F2L0W0 (Residues: 1-295; Coverage: 100%)
Gene names: TUZN_1299, ilvE
Sequence domains: Amino-transferase class IV

Ligands and Environments


Cofactor: Ligand PLP 3 x PLP
1 bound ligand:
Ligand PY5 3 x PY5
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P3212
Unit cell:
a: 93.46Å b: 93.46Å c: 213.17Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.187 0.234
Expression system: Escherichia coli

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