› 6tht

X-ray diffraction
1.14Å resolution

High resolution crystal structure of a Leaf-branch compost cutinase quintuple variant

Released:
DOI: 10.2210/pdb6tht/pdb
Model geometry
Fit model/data
PDB entry 6tht coloured by chain, front view.
PDB entry 6tht coloured by chain, side view.
PDB entry 6tht coloured by chain, top view.
Source organism: uncultured bacterium
Primary publication:
An engineered PET depolymerase to break down and recycle plastic bottles.
Tournier V, Topham CM, Gilles A, David B, Folgoas C, Moya-Leclair E, Kamionka E, Desrousseaux ML, Texier H, Gavalda S, Cot M, Guémard E, Dalibey M, Nomme J, Cioci G, Barbe S, Chateau M, André I, Duquesne S, Marty A
Nature 580 216-219 (2020)
PMID: 32269349

Function and Biology Details

Reactions catalysed: 
(Ethylene terephthalate)(n) + H(2)O = (ethylene terephthalate)(n-1) + ethylene terephthalate
Cutin + H(2)O = cutin monomers
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124660 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leaf-branch compost cutinase Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 27.65 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G9BY57 (Residues: 36-293; Coverage: 100%)
Sequence domains: Alpha/beta hydrolase family

Ligands and Environments

3 bound ligands:
Ligand IMD 1 x IMD
Ligand GOL 1 x GOL
Ligand CIT 1 x CIT
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P63
Unit cell:
a: 109.413Å b: 109.413Å c: 35.183Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.114 0.113 0.136
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

96 review citations

Biocatalysis: Enzymatic Synthesis for Industrial Applications.
Wu et al. (2021)
95 more

11 mentions without citation

Mechanism-Based Design of Efficient PET Hydrolases.
Wei et al. (2022)
10 more