› 6tiu

X-ray diffraction
3.57Å resolution

DROSOPHILA GTP-TUBULIN Y222F MUTANT

Released:
DOI: 10.2210/pdb6tiu/pdb
Model geometry
Fit model/data
PDB entry 6tiu coloured by chain, front view.
PDB entry 6tiu coloured by chain, side view.
PDB entry 6tiu coloured by chain, top view.
Source organisms:
Primary publication:
GTP-dependent formation of straight tubulin oligomers leads to microtubule nucleation.
Ayukawa R, Iwata S, Imai H, Kamimura S, Hayashi M, Ngo KX, Minoura I, Uchimura S, Makino T, Shirouzu M, Shigematsu H, Sekimoto K, Gigant B, Muto E
OpenAccess logo J Cell Biol 220 (2021)
PMID: 33544140

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-139089 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha-1 chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 450 amino acids
Theoretical weight: 49.99 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P06603 (Residues: 1-450; Coverage: 100%)
Gene names: CG1913, alphaTub84B, tubA84B
Sequence domains:
Tubulin beta-1 chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 447 amino acids
Theoretical weight: 50.18 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q24560 (Residues: 1-447; Coverage: 100%)
Gene names: CG9277, betaTub56D
Sequence domains:
Stathmin-4 Chain: E

Ligands and Environments

4 bound ligands:
Ligand GTP 4 x GTP
Ligand MG 4 x MG
Ligand SO4 5 x SO4
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P212121
Unit cell:
a: 65.97Å b: 126.35Å c: 249.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.221 0.244
Expression systems:
  • Trichoplusia ni
  • Escherichia coli

Quick links

Citations

4 review citations

Regulation of microtubule dynamics, mechanics and function through the growing tip.
Gudimchuk et al. (2021)
3 more

1 mention without citation

GTP-dependent formation of straight tubulin oligomers leads to microtubule nucleation.
Ayukawa et al. (2021)