› 6tjj

X-ray diffraction
1.59Å resolution

Structure of Cerezyme at pH 4.6

Released:
DOI: 10.2210/pdb6tjj/pdb
PDB entry 6tjj coloured by chain, front view.
PDB entry 6tjj coloured by chain, side view.
PDB entry 6tjj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A baculoviral system for the production of human β-glucocerebrosidase enables atomic resolution analysis.
Rowland RJ, Wu L, Liu F, Davies GJ
Acta Crystallogr D Struct Biol 76 565-580 (2020)
PMID: 32496218

Function and Biology Details

Reactions catalysed: 
D-glucosyl-N-acylsphingosine + H(2)O = D-glucose + N-acylsphingosine
D-galactosyl-N-acylsphingosine + H(2)O = D-galactose + N-acylsphingosine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137377 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lysosomal acid glucosylceramidase Chains: AAA, BBB
Molecule details ›
Chains: AAA, BBB
Length: 497 amino acids
Theoretical weight: 55.64 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P04062 (Residues: 40-536; Coverage: 100%)
Gene names: GBA, GBA1, GC, GLUC
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
5 bound ligands:
Ligand SO4 18 x SO4
Ligand ACT 16 x ACT
Ligand NAG 2 x NAG
Ligand K 1 x K
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2221
Unit cell:
a: 110.07Å b: 285.865Å c: 91.936Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.215 0.245
Expression system: Cricetulus griseus

Quick links

Citations

1 review citation

Sterylglucosides in Fungi.
Pereira de Sa et al. (2022)
3 other articles

1 mention without citation

A baculoviral system for the production of human β-glucocerebrosidase enables atomic resolution analysis.
Rowland et al. (2020)