› 6tjk

X-ray diffraction
1.56Å resolution

Crystal Structure of Recombinant GBA in Complex with Bis-Tris Propane.

Released:
DOI: 10.2210/pdb6tjk/pdb
PDB entry 6tjk coloured by chain, front view.
PDB entry 6tjk coloured by chain, side view.
PDB entry 6tjk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A baculoviral system for the production of human β-glucocerebrosidase enables atomic resolution analysis.
Rowland RJ, Wu L, Liu F, Davies GJ
Acta Crystallogr D Struct Biol 76 565-580 (2020)
PMID: 32496218

Function and Biology Details

Reactions catalysed: 
D-glucosyl-N-acylsphingosine + H(2)O = D-glucose + N-acylsphingosine
D-galactosyl-N-acylsphingosine + H(2)O = D-galactose + N-acylsphingosine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137377 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lysosomal acid glucosylceramidase Chains: AAA, BBB
Molecule details ›
Chains: AAA, BBB
Length: 497 amino acids
Theoretical weight: 55.66 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P04062 (Residues: 40-536; Coverage: 100%)
Gene names: GBA, GBA1, GC, GLUC
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
4 bound ligands:
Ligand SO4 10 x SO4
Ligand NAG 3 x NAG
Ligand EDO 43 x EDO
Ligand B3P 2 x B3P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 52.744Å b: 156.222Å c: 68.264Å
α: 90° β: 102.473° γ: 90°
R-values:
R R work R free
0.166 0.165 0.198
Expression system: Trichoplusia ni

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Citations

1 review citation

Sterylglucosides in Fungi.
Pereira de Sa et al. (2022)
2 other articles

2 mentions without citation

A baculoviral system for the production of human β-glucocerebrosidase enables atomic resolution analysis.
Rowland et al. (2020)
1 more