› 6tlc

X-ray diffraction
2.9Å resolution

Unphosphorylated human STAT3 in complex with MS3-6 monobody

Released:
DOI: 10.2210/pdb6tlc/pdb
PDB entry 6tlc coloured by chain, front view.
PDB entry 6tlc coloured by chain, side view.
PDB entry 6tlc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Selective inhibition of STAT3 signaling using monobodies targeting the coiled-coil and N-terminal domains.
La Sala G, Michiels C, Kükenshöner T, Brandstoetter T, Maurer B, Koide A, Lau K, Pojer F, Koide S, Sexl V, Dumoutier L, Hantschel O
Nat Commun 11 4115 (2020)
PMID: 32807795

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154182 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Signal transducer and activator of transcription 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 598 amino acids
Theoretical weight: 68.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P40763 (Residues: 127-722; Coverage: 77%)
Gene names: APRF, STAT3
Sequence domains:
Monobody Chains: C, D
Molecule details ›
Chains: C, D
Length: 95 amino acids
Theoretical weight: 10.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P41212
Unit cell:
a: 111.31Å b: 111.31Å c: 483.467Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.246 0.281
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The Multifaced Role of STAT3 in Cancer and Its Implication for Anticancer Therapy.
Tolomeo et al. (2021)
5 more

23 mentions without citation

The JAK-STAT pathway at 30: Much learned, much more to do.
Philips et al. (2022)
22 more