› 6tn1

X-ray diffraction
0.98Å resolution

Unliganded Crystal Structure of Recombinant GBA

Released:
DOI: 10.2210/pdb6tn1/pdb
PDB entry 6tn1 coloured by chain, front view.
PDB entry 6tn1 coloured by chain, side view.
PDB entry 6tn1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A baculoviral system for the production of human β-glucocerebrosidase enables atomic resolution analysis.
Rowland RJ, Wu L, Liu F, Davies GJ
Acta Crystallogr D Struct Biol 76 565-580 (2020)
PMID: 32496218

Function and Biology Details

Reactions catalysed: 
D-glucosyl-N-acylsphingosine + H(2)O = D-glucose + N-acylsphingosine
D-galactosyl-N-acylsphingosine + H(2)O = D-galactose + N-acylsphingosine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137377 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lysosomal acid glucosylceramidase Chain: AAA
Molecule details ›
Chain: AAA
Length: 497 amino acids
Theoretical weight: 55.66 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P04062 (Residues: 40-536; Coverage: 100%)
Gene names: GBA, GBA1, GC, GLUC
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
5 bound ligands:
Ligand GLN 1 x GLN
Ligand NAG 1 x NAG
Ligand EDO 7 x EDO
Ligand FMT 17 x FMT
Ligand MG 8 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P1
Unit cell:
a: 44.451Å b: 46.176Å c: 64.209Å
α: 86.04° β: 75.191° γ: 82.782°
R-values:
R R work R free
0.112 0.112 0.128
Expression system: Trichoplusia ni

Quick links

Citations

1 review citation

Sterylglucosides in Fungi.
Pereira de Sa et al. (2022)
3 other articles

2 mentions without citation

Structural insight into FANCI-FANCD2 monoubiquitination.
Li et al. (2020)
1 more