› 6tnh

X-ray diffraction
2.21Å resolution

Deoxyguanylosuccinate synthase (DgsS) quaternary structure with AMPPcP, dGMP, Asp, Magnesium at 2.21 Angstrom resolution

Released:
DOI: 10.2210/pdb6tnh/pdb
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PDB entry 6tnh coloured by chain, front view.
PDB entry 6tnh coloured by chain, side view.
PDB entry 6tnh coloured by chain, top view.
Source organism: Vibrio phage phiVC8
Primary publication:
A third purine biosynthetic pathway encoded by aminoadenine-based viral DNA genomes.
Sleiman D, Garcia PS, Lagune M, Loc'h J, Haouz A, Taib N, Röthlisberger P, Gribaldo S, Marlière P, Kaminski PA
Science 372 516-520 (2021)
PMID: 33926955

Function and Biology Details

Reaction catalysed: 
ATP + dGMP + L-aspartate = ADP + phosphate + 2-amino-2'-deoxy-N(6)-((2S)-succino)adenylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124325 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N6-succino-2-amino-2'-deoxyadenylate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 363 amino acids
Theoretical weight: 40.6 KDa
Source organism: Vibrio phage phiVC8
Expression system: Escherichia coli
UniProt:
  • Canonical: G3FFN6 (Residues: 3-343; Coverage: 99%)
Gene names: phiVC8_p27, purZ
Sequence domains: Adenylosuccinate synthetase

Ligands and Environments

5 bound ligands:
Ligand SO4 5 x SO4
Ligand DGP 2 x DGP
Ligand ACP 2 x ACP
Ligand ASP 1 x ASP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P41212
Unit cell:
a: 91.64Å b: 91.64Å c: 189.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.228
Expression system: Escherichia coli

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Citations

5 review citations

Bacteriophage-Encoded DNA Polymerases-Beyond the Traditional View of Polymerase Activities.
Morcinek-Orłowska et al. (2022)
4 more

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