› 6tqa

X-ray diffraction
2.4Å resolution

X-ray structure of Roquin ROQ domain in complex with a UCP3 CDE2 SL RNA motif

Released:
DOI: 10.2210/pdb6tqa/pdb
Model geometry
Fit model/data
PDB entry 6tqa coloured by chain, front view.
PDB entry 6tqa coloured by chain, side view.
PDB entry 6tqa coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis for the recognition of transiently structured AU-rich elements by Roquin.
Binas O, Tants JN, Peter SA, Janowski R, Davydova E, Braun J, Niessing D, Schwalbe H, Weigand JE, Schlundt A
OpenAccess logo Nucleic Acids Res 48 7385-7403 (2020)
PMID: 32491174

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175775 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Roquin-1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 180 amino acids
Theoretical weight: 20.53 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4VGL6 (Residues: 147-326; Coverage: 16%)
Gene names: Gm551, Kiaa2025, Rc3h1
Sequence domains:
RNA (5'-R(P*GP*GP*UP*GP*CP*CP*UP*AP*AP*UP*AP*UP*UP*UP*AP*GP*GP*CP*AP*CP*(CCC))-3') Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 21 nucleotides
Theoretical weight: 6.75 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand CL 3 x CL
Ligand MG 1 x MG
1 modified residue:
Ligand CCC 4 x CCC

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 44.53Å b: 160.66Å c: 68.01Å
α: 90° β: 107.96° γ: 90°
R-values:
R R work R free
0.2 0.197 0.259
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

The Role of MicroRNAs in Development and Function of Regulatory T Cells - Lessons for a Better Understanding of MicroRNA Biology.
Kunze-Schumacher et al. (2020)
1 more

1 mention without citation

Structural basis for the recognition of transiently structured AU-rich elements by Roquin.
Binas et al. (2020)