› 6trn

X-ray diffraction
1.35Å resolution

Crystal structure of HLA A2*01-AVYDGREHTV

Released:
DOI: 10.2210/pdb6trn/pdb
PDB entry 6trn coloured by chain, front view.
PDB entry 6trn coloured by chain, side view.
PDB entry 6trn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
T cell receptor interactions with human leukocyte antigen govern indirect peptide selectivity for the cancer testis antigen MAGE-A4.
Coles CH, McMurran C, Lloyd A, Hock M, Hibbert L, Raman MCC, Hayes C, Lupardus P, Cole DK, Harper S
J Biol Chem 295 11486-11494 (2020)
PMID: 32532817

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53Z42 (Residues: 25-300; Coverage: 81%)
Gene name: HLA-A
Sequence domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Melanoma-associated antigen 4 Chain: C
Molecule details ›
Chain: C
Length: 10 amino acids
Theoretical weight: 1.15 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P43358 (Residues: 230-239; Coverage: 3%)
Gene names: MAGE4, MAGEA4

Ligands and Environments

1 bound ligand:
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P21
Unit cell:
a: 55.97Å b: 80.69Å c: 58.66Å
α: 90° β: 115.13° γ: 90°
R-values:
R R work R free
0.163 0.161 0.2
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

6 review citations

TCR Recognition of Peptide-MHC-I: Rule Makers and Breakers.
Szeto et al. (2020)
5 more

3 mentions without citation

In vivo incorporation of unnatural amino acids to probe structure, dynamics, and ligand binding in a large protein by nuclear magnetic resonance spectroscopy.
Cellitti et al. (2008)
2 more